Rat IL-17A Recombinant Protein

Catalog Number:: RP0457R
Application:: Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:: Rattus norvegicus (Norway rat)

Product Description

Rat IL-17A (catalog RP0457R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.0 kDa, 133 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-17 signaling and inflammatory responses (including Th17-mediated immunity and cytokine induction); typical experimental uses include cell-culture stimulation and dosing studies, signaling and gene-expression assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Properties/Specifications

Amino Acid Sequence	AVLIPQSSVC PNAEANNFLQ NVKVNLKVLN SLSSKASSRR PSDYLNRSTS PWTLSRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPEKCPFT FRVEKMLVGV GCTCVSSIVR HAS (133)
Endotoxin	Naturally endotoxin-free
Form	Lyophilized
Storage Conditions	-20°C
Molecular Weight	15.0 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Affinity Tag	None
aminoAcids	133
Carrier Protein	None
Expression System	Yeast
Form	Lyophilized
Country Of Origin	USA

Rat IL-17A (Interleukin-17A) is a pro-inflammatory cytokine belonging to the IL-17 family that plays a central role in host defense and immune-mediated inflammation in rats (Rattus norvegicus). IL-17A is produced primarily by Th17 CD4⁺ T cells, as well as by γδ T cells and innate lymphoid cells, in response to microbial infection and inflammatory stimulation. It acts on epithelial, endothelial, and stromal cells to induce expression of pro-inflammatory cytokines (such as IL-6 and TNF-α), chemokines (including CXCL1 and CXCL2), and antimicrobial peptides, thereby promoting neutrophil recruitment and strengthening mucosal barrier immunity. In healthy rats, basal IL-17A expression is generally low but increases during bacterial and fungal infections, autoimmune responses, and inflammatory challenge. Rat IL-17A has been implicated in experimental models of rheumatoid arthritis, experimental autoimmune encephalomyelitis (EAE), inflammatory bowel disease, asthma, and cardiovascular inflammation. In biomedical research, rat IL-17A serves as an important biomarker and mechanistic mediator of Th17-driven immune activation, neutrophilic inflammation, and autoimmune pathology in translational disease models.