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Canine IFN alpha Recombinant Protein
- Catalog Number:
- RP0463D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine IFN-α (catalog RP0463D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.0 kDa, 164 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study type I interferon signaling and antiviral immune responses (including regulation of innate immunity and gene expression); typical experimental uses include cell-culture stimulation and antiviral assays, signaling and gene-expression studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | CHLPDTHGLR NWRVLTLLGQ MRRLSAGSCD HYTNDFAFPK ELFDGQRLQE AQALSVVHVM TQKVFHLFCP DTSSAPWNMT LLEELCSGLS EQLDDLEACP LQEAGLAETP LMHEDSTLRT YFQRISLYLQ DRNHSPCAWE MVRAEIGRSF FSSTILQERI RRRK (164) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 19.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Interferon-Alpha (IFN-α) is a type I interferon produced primarily by plasmacytoid dendritic cells and virus-infected cells in dogs (Canis lupus familiaris), where it plays a central role in early innate antiviral immunity. Upon binding to the type I interferon receptor complex (IFNAR1/IFNAR2), canine IFN-α activates the JAK/STAT signaling pathway, inducing interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. In canine health, IFN-α is critically involved in host defense against viral infections such as canine parvovirus, canine distemper virus, canine influenza virus, and canine adenovirus, and altered interferon responses can influence disease severity and viral persistence. Recombinant interferons have also been explored for therapeutic use in viral and immune-mediated conditions. Importantly, dogs serve as valuable spontaneous animal models for human infectious diseases, autoimmune disorders (such as immune-mediated hemolytic anemia), and certain cancers, where type I interferon signaling contributes to antiviral defense, tumor immune surveillance, and immunomodulation. Characterization of canine IFN-α therefore supports vaccine development, antiviral strategy research, oncology studies, and comparative investigations of interferon biology relevant to both veterinary medicine and translational human health research.
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