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Canine BAFF Recombinant Protein
- Catalog Number:
- RP0465D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine BAFF (catalog RP0465D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.1 kDa, 152 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study BAFF/TNFSF13B signaling and B-cell biology (including survival, maturation, and antibody production); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and survival assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AIQGPEETVT QDCLQLIADS DTPTIRKGAY TFVPWLLSFK RGRALEEKEN KILVKEAGYF FIYSQVLYTD NTFAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PREDAKISRD GDGTFFGALK LL (152) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.1 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine BAFF (B cell-Activating Factor), also known as BLyS or TNFSF13B, is a member of the tumor necrosis factor (TNF) superfamily and plays a critical role in B cell development, survival, and humoral immunity in dogs (Canis lupus familiaris). BAFF is produced primarily by monocytes, macrophages, dendritic cells, and certain stromal cells, and it signals through the receptors BAFF-R (TNFRSF13C), TACI (TNFRSF13B), and BCMA (TNFRSF17) to promote B cell maturation, peripheral B cell survival, immunoglobulin class switching, and maintenance of long-lived plasma cells. In healthy dogs, BAFF supports normal antibody production and immune homeostasis; however, dysregulated or elevated BAFF expression may contribute to pathological B cell activation and autoantibody production. In veterinary and comparative medicine research, canine BAFF is particularly relevant in naturally occurring autoimmune diseases such as immune-mediated hemolytic anemia (IMHA), immune-mediated thrombocytopenia (IMTP), systemic lupus erythematosus (SLE)-like syndromes, and chronic inflammatory conditions, as well as in B cell-derived malignancies including lymphoma and multiple myeloma. Measurement and characterization of canine BAFF provide valuable insight into B cell-mediated immune regulation, antibody-driven pathology, and potential therapeutic strategies targeting BAFF signaling in both veterinary patients and translational immunology research.
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