Canine CCL2 (MCP-1) Recombinant Protein

Catalog Number:: RP0466D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus familiaris (dog)

Product Description

Canine CCL2 (MCP-1) (catalog RP0466D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.9 kDa, 78 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL2/MCP-1 signaling and immune-cell chemotaxis (including monocyte recruitment and migration); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	QPDAIISPVT CCYTLTNKKI SIQRLASYKR VTSSKCPKEA VIFKTVLNKE ICADPKQKWV QDSMAHLDKK SQTQTAKP (78)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	8.9 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that plays a key role in recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in dogs (Canis lupus familiaris). CCL2 signals primarily through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, inflammation, or tissue injury. In canine health, CCL2 is particularly important in inflammatory and immune-mediated diseases such as canine osteoarthritis, chronic kidney disease, inflammatory bowel disease, and canine atopic dermatitis, where elevated CCL2 contributes to macrophage recruitment and sustained tissue inflammation. It is also implicated in infectious diseases and tumor biology, including canine lymphoma and osteosarcoma, where CCL2 can influence tumor-associated macrophage infiltration and tumor microenvironment remodeling. While CCL2-mediated immune cell trafficking supports pathogen defense and tissue repair, excessive or persistent expression can drive chronic inflammation and tissue damage. As a biomarker of monocyte/macrophage recruitment and inflammatory activation, canine CCL2 is widely used in studies of host-pathogen interactions, immune regulation, and disease progression. Because dogs serve as important spontaneous models for human inflammatory diseases and cancer, characterization of canine CCL2 supports translational research into chemokine signaling and development of targeted anti-inflammatory therapies.

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