Canine IL-1 alpha Recombinant Protein

Catalog Number:: RP0486D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog)

Product Description

Canine IL-1α (catalog RP0486D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.2 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-1 signaling and inflammatory responses (including activation of immune and stromal cells); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SVAYNFHNNE KYNYIRIIKS QFILNDNLNQ SIVRQTGGNY LMTAALQNLD DAVKFDMGAY TSEDSKLPVT LRISKTRLFV SAQNEDEPVL LKEMPETPKT IRDETNLLFF WERHGSKHYF KSVAQPKLFI ATQERKLVHM ARGQPSITDF RLLETQP (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	18.2 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family, which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra), and plays a key role in initiating and amplifying innate immune responses in dogs (Canis lupus familiaris). IL-1α is constitutively expressed in epithelial and barrier tissues and functions as an intracellular alarmin that is rapidly released upon cell injury or necrosis, triggering early inflammatory signaling. It binds to the IL-1 receptor type 1 (IL-1R1), activating NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and production of additional pro-inflammatory mediators. In canine health, IL-1α is particularly relevant in inflammatory skin diseases such as atopic dermatitis, wound healing responses, osteoarthritis, inflammatory bowel disease, and certain neoplastic conditions where tumor-associated inflammation contributes to disease progression. While essential for pathogen defense and tissue repair, excessive or chronic IL-1α signaling can exacerbate tissue damage and perpetuate inflammation. As an early mediator of sterile and infectious inflammation, canine IL-1α serves as a biomarker of tissue injury and innate immune activation. In translational research, dogs are valuable spontaneous models for human dermatologic disease, osteoarthritis, inflammatory bowel disease, and cancer, and characterization of canine IL-1α supports comparative studies of cytokine-driven inflammation and development of targeted anti-inflammatory therapies relevant to both veterinary and human medicine.

Choose a size

Please choose a size to see price

RELATED MEDIA