Multi-species (Ovine, Caprine) IL-8 Recombinant Protein

Catalog Number:: RP0488V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Capra hircus (goat), Ovis aries (sheep), Ovis canadensis (bighorn sheep)

Ovine and Caprine IL-8 (catalog RP0488V) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.1 kDa, 79 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep and goat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-8 signaling and chemotaxis (including recruitment and activation of neutrophils); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	AVLSRMSTEL RCQCIKTHST PFHPKFIKEL RVIESGPHCE NSEIIVKLTN GKEVCLDPKE KWVQKVVQAF LKRAEKQDP (79)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	9.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Ovine and Caprine Interleukin-8 (IL-8), also known as CXCL8, is a highly conserved pro-inflammatory chemokine that plays a central role in innate immune responses in sheep (Ovis aries) and goats (Capra hircus), with the IL-8 protein sequence being identical between the two species. Produced by macrophages, epithelial cells, endothelial cells, and mammary gland cells in response to bacterial, viral, and inflammatory stimuli, IL-8 signals through CXCR1 and CXCR2 receptors to mediate neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Elevated IL-8 expression is associated with economically significant small ruminant diseases including mastitis, pneumonia, caseous lymphadenitis, enteric infections, foot rot, and parasitic infestations, where neutrophil-driven inflammation contributes to both pathogen clearance and tissue pathology. In the mammary gland, IL-8 is a key mediator of somatic cell recruitment during intramammary infection and serves as an early biomarker of mastitis severity and therapeutic response. In animal health and translational research, characterization of ovine/caprine IL-8 supports studies of mucosal immunity, respiratory disease, mammary gland inflammation, and host-pathogen interactions in small ruminants, while also providing comparative insight into neutrophil-mediated inflammatory mechanisms relevant to human respiratory and inflammatory diseases.

Choose a size

Please choose a size to see price

RELATED MEDIA