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Swine TWEAK (TNFSF12) Recombinant Protein
- Catalog Number:
- RP0489S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Phacochoerus africanus (Common warthog), Sus scrofa (pig)
- Swine TWEAK (TNFSF12) (catalog RP0489S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.3 kDa, 156 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TWEAK/TNFSF12 signaling and immune and tissue biology (including inflammation, apoptosis, and tissue remodeling); typical experimental uses include cell-culture stimulation and dosing studies, signaling and functional assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SAPKGRKPRA RRVIAAHYEV HPRPGQDGAQ AGVDGTVSGW EEAKINSSNP LRYDRQSGQF LVTRAGLYYL YCQVHFDEGK AVYLKLDLLV DDTLALRCLE EFSATAASSL GPQLRLCQVS GLLPLRPGSS LRIRTLPWAH LKAAPFLTYF GLFQVH (156) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.3 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine TWEAK (TNF-like Weak Inducer of Apoptosis, also known as TNFSF12) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), TRAIL, and RANKL that regulate immune signaling, apoptosis, and tissue remodeling. In pigs (Sus scrofa), TWEAK is produced primarily by immune cells such as macrophages, monocytes, dendritic cells, and T lymphocytes, and can also be expressed by endothelial cells and various tissue cells during inflammation or tissue injury. TWEAK exerts its biological effects mainly through binding to its receptor Fn14 (fibroblast growth factor-inducible 14, TNFRSF12A), activating intracellular signaling pathways including NF-κB and MAPK, which regulate cell proliferation, apoptosis, inflammatory cytokine production, and tissue remodeling. In swine physiology, the TWEAK-Fn14 signaling axis contributes to immune responses, tissue repair, and regulation of inflammatory processes following infection or cellular damage. Because pigs are widely used as large-animal models for human inflammatory disease, regenerative biology, and cardiovascular research, characterization of swine TWEAK supports studies of TNF superfamily signaling, immune regulation, and mechanisms of tissue injury and repair relevant to both veterinary medicine and translational biomedical research.
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