Ovine IFN gamma Recombinant Protein

Catalog Number:: RP0491V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Ovis aries (sheep)

Ovine IFN-γ (catalog RP0491V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.9 kDa, 143 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IFN-γ signaling and immune responses (including macrophage activation and Th1 immunity); typical experimental uses include cell-culture stimulation and dosing studies, signaling and gene-expression assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	QGPFFKEIEN LKEYFNASNP DVAKGGPLFS EILKNWKEES DKKIIQSQIV SFYFKLFENL KDNQVIQRSM DIIKQDMFQK FLNGSSEKLE DFKRLIQIPV DDLQIQRKAI NELIKVMNDL SPKSNLRKRK RSQNLFRGRR ASM (143)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	16.9 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Ovine Interferon-γ (IFN-γ) is a type II interferon cytokine that plays a central role in cell-mediated immunity and regulation of antimicrobial and inflammatory responses in sheep (Ovis aries). IFN-γ is produced primarily by activated T helper 1 (Th1) cells, cytotoxic T lymphocytes, and natural killer (NK) cells following stimulation by intracellular pathogens or immune signaling molecules such as IL-12 and IL-18. Ovine IFN-γ binds to the interferon-γ receptor (IFNGR1/IFNGR2) expressed on many immune and non-immune cells, activating intracellular signaling through the JAK-STAT pathway, particularly STAT1, which induces the expression of numerous interferon-stimulated genes involved in antigen presentation, macrophage activation, and antimicrobial defense. In sheep, IFN-γ is critical for activation of macrophages, enhancement of MHC class I and II expression, and control of intracellular pathogens including viruses, bacteria, and parasites. Ovine IFN-γ is widely studied in veterinary immunology and livestock disease research; for example, IFN-γ responses are commonly measured in studies of Mycobacterium avium subsp. paratuberculosis infection (Johne's disease), where IFN-γ production by antigen-stimulated lymphocytes is used as an indicator of cell-mediated immune responses and early infection in sheep.

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