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Canine TWEAK (TNFSF12) Recombinant Protein
- Catalog Number:
- RP0655D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes vulpes (red fox)
- Canine TWEAK (TNFSF12) (catalog RP0655D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.2 kDa, 156 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog, dingo, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TWEAK/TNFSF12 signaling and immune and tissue biology (including inflammation, apoptosis, and tissue remodeling); typical experimental uses include cell-culture stimulation and dosing studies, signaling and functional assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | NASKGRKTRA RRAIAAHYEV HPQPGQDGGQ AGVDGTVSGW EEAKINSSNP LRYDRQSGEF IVTRAGLYYL YCQVHFDEGK AVYLKLDLLV DDALALRCLE EFSATAASSL GPQLRLCQVS GLLPLRPGSS LRIRTLPWAH LKAAPFLTYF GLFQVN (156) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine TWEAK (TNF-like Weak Inducer of Apoptosis, also known as TNFSF12) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), TRAIL, and RANKL that regulate immune signaling, apoptosis, and tissue remodeling. In dogs (Canis lupus familiaris), TWEAK is produced primarily by immune cells such as macrophages, monocytes, and T lymphocytes, and can also be expressed by endothelial cells and other tissue cells during inflammation or tissue injury. TWEAK exerts its biological effects mainly through binding to its receptor Fn14 (fibroblast growth factor-inducible 14, TNFRSF12A), activating intracellular signaling pathways including NF-κB, MAPK, and other inflammatory signaling cascades that regulate cell survival, proliferation, apoptosis, and cytokine production. In canine physiology, the TWEAK-Fn14 signaling axis is involved in inflammatory responses, tissue repair, and regulation of immune cell activity, particularly during tissue injury or infection. Dysregulation of this pathway may contribute to chronic inflammation, tissue remodeling, and cancer-associated processes. Because TWEAK signaling plays important roles in inflammation, immune regulation, and tissue regeneration, canine TWEAK is of interest in veterinary immunology, inflammatory disease research, and oncology, helping to improve understanding of cytokine-mediated signaling and tissue repair mechanisms in dogs.
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