Ovine IL-1 beta Recombinant Protein

Catalog Number:: RP0656V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Ovis aries (sheep), Pseudois nayaur (bharal)

Product Description

Ovine IL-1β (catalog RP0656V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.7 kDa, 153 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep and bharal. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-1 signaling and inflammatory responses (including activation of immune cells and cytokine production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	AAVQSVKCKL QDREQKSLVL DSPCVLKALH LLSQEMSREV VFCMSFVQGE ERDNKIPVAL GIRDKNLYLS CVKKGDTPTL QLEEVDPKVY PKRNMEKRFV FYKTEIKNTV EFESVLYPNW YISTSQIEEK PVFLGRFRGG QDITDFRMET LSP (153)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Ovine Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in sheep (Ovis aries) in response to infection, tissue injury, or inflammatory stimuli. Synthesized as an inactive precursor (pro-IL-1β), it is cleaved into its active form by caspase-1 within inflammasome complexes-most notably the NLRP3 inflammasome-and signals through the IL-1 receptor type 1 (IL-1R1), activating NF-κB and MAPK pathways that promote fever, leukocyte recruitment, endothelial activation, and acute-phase protein production. In ovine health, IL-1β plays a central role in inflammatory conditions associated with mastitis, pneumonia, foot rot, enteric infections, parasitic infestations, and viral diseases such as ovine progressive pneumonia (maedi-visna), where excessive IL-1β production can contribute to tissue damage and chronic inflammation. Elevated IL-1β expression is also observed in the mammary gland during intramammary infection and serves as a marker of disease severity. As a biomarker of innate immune activation, ovine IL-1β is important for evaluating vaccine responses and immune status in flock management. In veterinary and translational research, characterization of ovine IL-1β supports studies of inflammasome biology, host-pathogen interactions in small ruminants, inflammatory regulation, and development of targeted anti-inflammatory strategies relevant to both animal health and comparative immunology.

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