Canine CXCL10 (IP-10) Recombinant Protein

Catalog Number:: RP0754D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog)

Product Description

Canine CXCL10 (IP-10) (catalog RP0754D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.6 kDa, 77 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL10/IP-10 signaling and immune-cell chemotaxis (including recruitment of activated T cells and NK cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	IPLSRTIRCT CIKISDQPVN LRSLEKIEMI PASPSCPHVE IIATMKKSGE KRCLNPESKT IKSLVKAISK KRSRRSP (77)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	8.6 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine CXCL10 (C-X-C motif chemokine ligand 10), also known as IP-10 (Interferon gamma-induced protein 10), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL9 (MIG) and CXCL11 (I-TAC) that regulate T-cell recruitment during antiviral and inflammatory immune responses. In dogs (Canis lupus familiaris), CXCL10 is produced by macrophages, dendritic cells, epithelial cells, endothelial cells, and fibroblasts in response to interferon-γ (IFN-γ), viral infection, or inflammatory cytokines. CXCL10 signals primarily through the chemokine receptor CXCR3, which is expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and activation of these cells at sites of infection or inflammation. In canine health, CXCL10 contributes to immune responses against viral, bacterial, and parasitic pathogens, and elevated CXCL10 expression has been observed in inflammatory and immune-mediated diseases, including canine leishmaniasis, where recruitment of Th1-type T cells is important for parasite control. Dogs also serve as comparative animal models for human infectious and immune-mediated diseases, and canine leishmaniasis is widely studied as a model for human visceral leishmaniasis, where CXCL10-mediated recruitment of CXCR3⁺ T cells is associated with protective cell-mediated immunity and disease progression, making canine CXCL10 relevant for studies of host-pathogen interactions, immune regulation, and therapeutic development.

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