Equine IL-10 Recombinant Protein

Catalog Number:: RP0757E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus caballus (horse), Equus przewalskii (Przewalski's horse)

Product Description

Equine IL-10 (catalog RP0757E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.6 kDa, 160 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse and Przewalski's horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-10 signaling and immune regulation (including anti-inflammatory responses and cytokine suppression); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SRDRGTQSEN SCTHFPTSLP HMLHELRAAF SRVKTFFQMK DQLDNMLLNG SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENHGPDIKEH VNSLGEKLKT LRVRLRRCHR FLPCENKSKA VEQVKSAFSK LQEKGVYKAM SEFDIFINYI EAYMTTKMKN (160)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	18.6 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Equine Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced by regulatory T cells (Tregs), Th2 cells, macrophages, dendritic cells, and certain B-cell populations in horses (Equus caballus), where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, TNF-α, and IFN-γ), reduce antigen presentation, and modulate Th1-type cell-mediated responses. In equine health, IL-10 is important in regulating inflammatory conditions such as equine asthma (recurrent airway obstruction), colitis, endotoxemia, laminitis, osteoarthritis, and chronic infections including equine herpesvirus and parasitic diseases, where balanced IL-10 expression helps prevent excessive tissue damage but may also contribute to immune suppression if overexpressed. The IL-10/pro-inflammatory cytokine ratio is often used as an indicator of immune status and disease progression. In veterinary and translational research, characterization of equine IL-10 supports studies of immune tolerance, vaccine responses, respiratory and gastrointestinal inflammation, and Th1/Th2 balance. Because horses serve as valuable large-animal models for human asthma, osteoarthritis, and systemic inflammatory conditions, investigation of equine IL-10 provides comparative insight into cytokine-mediated regulation of inflammation relevant to both equine and human health.

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