Human IL-2 Recombinant Protein

Catalog Number:: RP0758H
Application:: Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:: Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee)

Product Description

Human IL-2 (catalog RP0758H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.4 kDa, 133 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, gorilla, chimpanzee, and bonobo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-2 signaling and T-cell biology (including T-cell proliferation, activation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and activation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Properties/Specifications

Amino Acid Sequence	APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT (133)
Endotoxin	Naturally endotoxin-free
Form	Lyophilized
Storage Conditions	-20°C
Molecular Weight	15.4 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Affinity Tag	None
aminoAcids	133
Carrier Protein	None
Expression System	Yeast
Form	Lyophilized
Country Of Origin	USA

Human IL-2 (Interleukin-2) is a central T cell–derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis. IL-2 is produced primarily by activated CD4⁺ T helper cells and, to a lesser extent, by CD8⁺ T cells following antigen recognition and costimulatory signaling. It functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ/CD122, and the common γ chain/CD132) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support the development and maintenance of regulatory T cells (Tregs), which are essential for immune tolerance. In healthy individuals, circulating IL-2 levels are typically low but rise transiently during infection or immune activation. Dysregulated IL-2 signaling has been implicated in autoimmune diseases such as type 1 diabetes, multiple sclerosis, and systemic lupus erythematosus, as well as in immunodeficiency and chronic viral infections. Recombinant IL-2 has been used clinically as an immunotherapy for certain cancers (e.g., metastatic melanoma and renal cell carcinoma) and is being investigated at low doses to selectively expand Tregs in autoimmune and inflammatory disorders. In clinical and translational research, human IL-2 is a key biomarker and therapeutic target for modulating T cell–mediated immunity and immune tolerance.