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Rat CCL4 (MIP-1 beta) Recombinant Protein
- Catalog Number:
- RP0866R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat CCL4 (MIP-1β) (catalog RP0866R) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL4/MIP-1β signaling and immune-cell chemotaxis (including recruitment and activation of macrophages, NK cells, and T cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN (69) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in rats (Rattus norvegicus). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection, inflammation, or tissue injury. In rats, CCL4 is produced by activated macrophages, dendritic cells, T lymphocytes, epithelial cells, and endothelial cells in response to inflammatory stimuli such as microbial products and cytokines including TNF-α and IL-1β. Rat CCL4 is widely studied in experimental models of neuroinflammation, autoimmune disease, cardiovascular injury, and infectious disease, where chemokine-driven immune cell recruitment contributes to inflammatory signaling and tissue pathology. It has been implicated in models of neuropathic pain, stroke, and central nervous system injury, where CCL4-mediated leukocyte trafficking influences neuroimmune interactions and inflammatory responses in the brain and spinal cord. While CCL4-driven immune cell migration supports host defense and tissue repair, excessive or prolonged expression may contribute to chronic inflammation and tissue damage. Because rats are widely used in neuroscience, pharmacology, and inflammatory disease research, characterization of rat CCL4 supports investigations into CCR5-mediated immune cell trafficking, inflammatory signaling pathways, and host-pathogen interactions relevant to human disease.
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