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Canine CCL4 (MIP-1 beta) Recombinant Protein
- Catalog Number:
- RP0868D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine CCL4 (MIP-1β) (catalog RP0868D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog, dingo, Arctic fox, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL4/MIP-1β signaling and immune-cell chemotaxis (including recruitment and activation of macrophages, NK cells, and T cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APMGSDPPTA CCFSYTLRKI PRNFVADYFE TSSLCSQPAV VFQTRRGRQV CANPSEPWVQ EYMDDLELN (69) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine that plays an important role in recruitment and activation of immune cells during inflammatory and antiviral immune responses in dogs (Canis lupus familiaris). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In dogs, CCL4 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL4-mediated leukocyte recruitment contributes to host defense in infectious diseases such as canine distemper virus, parvovirus infection, and bacterial infections, where coordinated immune cell trafficking supports pathogen clearance. CCL4 is also implicated in chronic inflammatory and immune-mediated conditions, including dermatitis and inflammatory bowel disease, as well as in canine cancers such as lymphoma, where chemokine signaling can influence immune cell infiltration within the tumor microenvironment. While CCL4-driven leukocyte migration is important for immune activation and pathogen defense, excessive or prolonged expression may contribute to tissue inflammation and damage. As a regulator of immune cell trafficking and inflammatory signaling, canine CCL4 is important for studies of host-pathogen interactions, immune regulation, and inflammatory disease mechanisms relevant to both veterinary medicine and comparative immunology.
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