Bovine GM-CSF Recombinant Protein

Catalog Number:: RP0871B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Bovine GM-CSF (catalog RP0871B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.3 kDa, 126 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and myeloid-cell biology (including proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	APTRPPNTAT RPWQHVDAIK EALSLLNHSS DTDAVMNDTE VVSEKFDSQE PTCLQTRLKL YKNGLQGSLT SLMGSLTMMA THYEKHCPPT PETSCGTQFI SFKNFKEDLK EFLFIIPFDC WEPAQK (126)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	14.3 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In cattle (Bos taurus), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and other stromal cells in response to infection, inflammatory cytokines, or immune stimulation. Bovine GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, as well as enhance their functional activity in innate and adaptive immune responses. In bovine health, GM-CSF contributes to host defense against bacterial infections, particularly by regulating macrophage and neutrophil activity in tissues such as the mammary gland and respiratory tract. Bovine GM-CSF has also been studied in animal models of bovine tuberculosis caused by Mycobacterium bovis, where GM-CSF influences macrophage activation and antigen-presenting cell development, helping researchers understand cell-mediated immunity and vaccine responses to mycobacterial infections relevant to both livestock health and human tuberculosis research.

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