Ovine TNF alpha Recombinant Protein

Catalog Number:: RP0902V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Ovis aries (sheep), Ovis canadensis (bighorn sheep)

Product Description

Ovine TNF-α (catalog RP0902V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.2 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep and bighorn sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TNF-α signaling and inflammatory responses (including regulation of immune-cell activation, apoptosis, and cytokine production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	LRSSSQASNN KPVAHVVANI SAPGQLRWGD SYANALMANG VELKDNQLVV PTDGLYLIYS QVLFRGHGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETLEGAE AKPWYEPIYQ GGVFQLEKGD RLSAEINLPE YLDYAESGQV YFGIIAL (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.2 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Ovine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in sheep (Ovis aries). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In sheep, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In ovine health, TNF-α contributes to immune responses against bacterial, viral, and parasitic infections, including diseases such as ovine mastitis, respiratory infections, and parasitic infestations, where it promotes macrophage activation and inflammatory defense mechanisms. While TNF-α is essential for pathogen clearance, excessive or prolonged TNF signaling can contribute to chronic inflammation and tissue damage. Because TNF-α is a key mediator of inflammatory responses, ovine TNF-α is studied in veterinary immunology, infectious disease research, and inflammatory disease models, contributing to improved understanding of immune regulation and disease resistance in sheep.

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