Canine GM-CSF Recombinant Protein

Catalog Number:: RP0918D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog)

Canine GM-CSF (catalog RP0918D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.2 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and myeloid-cell biology (including proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK (127)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	14.2 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Canine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In dogs (Canis lupus familiaris), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and other stromal cells in response to infection, inflammatory cytokines, or immune stimulation. Canine GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, as well as enhance their functional activity in host defense and antigen presentation. In canine health, GM-CSF contributes to innate and adaptive immune responses during infection and inflammation, particularly through regulation of macrophage and neutrophil activity. Dogs are also valuable comparative animal models for human cancer immunology, and GM-CSF has been studied in canine melanoma models, where GM-CSF-expressing tumor vaccines have been used to stimulate dendritic cell activation and anti-tumor immune responses, providing insights relevant to both veterinary oncology and human cancer immunotherapy.

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