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Human GM-CSF Recombinant Protein
- Catalog Number:
- RP0919H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Homo sapiens (human)
- Human GM-CSF (catalog RP0919H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.5 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and myeloid-cell biology (including proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE (127) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 14.5 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In humans (Homo sapiens), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Human GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, and enhance their antigen-presenting and inflammatory functions. In human health, GM-CSF plays an important role in host defense against infections, regulation of inflammatory responses, and development of immune cells involved in adaptive immunity. Dysregulation of GM-CSF signaling has been associated with inflammatory and autoimmune diseases, including rheumatoid arthritis and pulmonary alveolar proteinosis. Because of its ability to stimulate immune cell development and activation, GM-CSF is widely studied and used in clinical immunotherapy and vaccine research, including GM-CSF-based cancer vaccines designed to enhance dendritic cell activation and anti-tumor immune responses.
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