Rabbit APRIL Recombinant Protein

Catalog Number:
RP0928U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit APRIL (TNFSF13) (catalog RP0928U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.5 kDa, 146 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study APRIL/TNFSF13 signaling and B-cell biology (including plasma-cell survival and antibody production); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and survival assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceALPTQKQKKK RSLLHLVPIN ITSKEDSDVT EVMWQPALRR GRGLEAQGYV VRVWDTGVYL LYSQVLFHDV TFTMGQVVSR EGQGRQETLF RCVCSMPSDP DRAYNSCYSA GVFHLHQGDI LSVVIPRARA KFSLSPHGTF LGFVKL (146)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily that plays a key role in B cell survival, plasma cell maintenance, and regulation of humoral immunity in rabbits (Oryctolagus cuniculus). APRIL is produced primarily by monocytes, macrophages, dendritic cells, and certain epithelial and stromal cells, and it signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17) to promote B cell maturation, immunoglobulin class switching, and the persistence of long-lived antibody-secreting plasma cells in lymphoid tissues and bone marrow. In rabbits, which are widely used for polyclonal and monoclonal antibody production, APRIL contributes to robust antibody responses and maintenance of humoral immunity, including mucosal IgA production. Dysregulated APRIL expression may be associated with chronic inflammation, autoimmune-like conditions, or B cell–related proliferative disorders in experimental models. In biomedical and translational research, rabbit APRIL is of particular interest in studies of vaccine immunogenicity, antibody production, mucosal immunity, and comparative B cell biology, where understanding APRIL-mediated signaling provides insight into regulation of antibody responses and potential strategies to modulate humoral immunity.

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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