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Canine Erythropoietin (EPO) Recombinant Protein
- Catalog Number:
- RP0931D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine Erythropoietin (EPO) (catalog RP0931D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.4 kDa, 166 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study EPO signaling and erythropoiesis (including regulation of red blood cell production and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APPRLICDSR VLERYILEAR EAENVTMGCA QGCSFSENIT VPDTKVNFYT WKRMDVGQQA LEVWQGLALL SEAILRGQAL LANASQPSET PQLHVDKAVS SLRSLTSLLR ALGAQKEAMS LPEEASPAPL RTFTVDTLCK LFRIYSNFLR GKLTLYTGEA CRRGDR (166) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 18.4 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Erythropoietin (EPO) is a glycoprotein hormone belonging to the type I cytokine family that regulates erythropoiesis, the production of red blood cells. In dogs (Canis lupus familiaris), erythropoietin is produced primarily by peritubular interstitial cells of the kidney, with smaller amounts produced in the liver, in response to hypoxia or decreased oxygen-carrying capacity of the blood. Canine EPO binds to the erythropoietin receptor (EPOR) on erythroid progenitor cells in the bone marrow, activating intracellular signaling pathways such as JAK2-STAT5, PI3K-AKT, and MAPK, which promote the survival, proliferation, and differentiation of erythroid precursor cells into mature red blood cells. In canine health, erythropoietin is essential for maintaining normal hematocrit levels and adequate oxygen delivery to tissues, and insufficient EPO production is a major contributor to anemia associated with chronic kidney disease (CKD) in dogs. Dogs are also used as comparative animal models for renal anemia, and studies of canine erythropoietin have helped improve understanding of the pathophysiology of anemia of chronic kidney disease and the therapeutic use of recombinant erythropoietin or erythropoiesis-stimulating agents, benefiting both veterinary medicine and human nephrology.
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