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Equine Erythropoietin (EPO) Recombinant Protein
- Catalog Number:
- RP0932E
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Equus caballus (horse), Equus przewalskii (Przewalski's horse)
- Equine Erythropoietin (EPO) (catalog RP0932E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.3 kDa, 166 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse and Przewalski's horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study EPO signaling and erythropoiesis (including regulation of red blood cell production and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APPRLICDSR VLERYILEAR EAENVTMGCA EGCSFGENVT VPDTKVNFYS WKRMEVEQQA VEVWQGLALL SEAILQGQAL LANSSQPSET LRLHVDKAVS SLRSLTSLLR ALGAQKEAIS PPDAASAAPL RTFAVDTLCK LFRIYSNFLR GKLKLYTGEA CRRGDR (166) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 18.3 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Equine Erythropoietin (EPO) is a glycoprotein hormone belonging to the type I cytokine family that regulates erythropoiesis, the production of red blood cells. In horses (Equus caballus), erythropoietin is produced primarily by peritubular interstitial cells in the kidney, with smaller amounts synthesized in the liver, in response to hypoxia or reduced oxygen-carrying capacity of the blood. Equine EPO binds to the erythropoietin receptor (EPOR) on erythroid progenitor cells in the bone marrow, activating intracellular signaling pathways such as JAK2-STAT5, PI3K-AKT, and MAPK, which promote the survival, proliferation, and differentiation of erythroid precursor cells into mature red blood cells. In equine physiology, erythropoietin plays a crucial role in maintaining adequate oxygen delivery to tissues, which is particularly important for athletic performance and endurance in horses. Dysregulation of EPO production can contribute to anemia associated with chronic disease or kidney dysfunction. Horses are also used as comparative models for studying exercise physiology and erythropoiesis, and research on equine erythropoietin has contributed to understanding red blood cell regulation during intense exercise, hypoxia, and blood disorders, as well as the detection of recombinant EPO misuse in equine sports.
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