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Swine CD40 Ligand (TNFSF5) Recombinant Protein
- Catalog Number:
- RP0936S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Phacochoerus africanus (Common warthog), Sus scrofa (pig)
- Swine CD40 Ligand (CD40L, CD154, TNFSF5) (catalog RP0936S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.0 kDa, 149 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig and warthog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CD40L/CD154 signaling and immune-cell activation (including regulation of B-cell proliferation, antibody production, and T-cell interactions); typical experimental uses include cell-culture stimulation and dosing studies, activation and signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MHKGDQDPQI AAHVISEASS KTASVLQWAP KGYYTLSTNL VTLENGRQLA VKRQGIYYIY AQVTFCSNRD AAGQAPFIAS LCLRSPSGSE RILLRAANTH SSSKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL (149) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine CD40 Ligand (CD40L), also known as CD154 and encoded by the CD40LG gene, is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and cell-cell signaling. In pigs (Sus scrofa), CD40L is primarily expressed on activated CD4⁺ T lymphocytes, and can also be detected on platelets and other activated immune cells following antigen stimulation. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, triggering intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, proliferation, immunoglobulin class switching, germinal center formation, and the development of memory B cells. Through these mechanisms, swine CD40L plays a key role in T cell-dependent antibody responses and regulation of adaptive immunity. In swine health, the CD40-CD40L signaling pathway contributes to immune responses against viral and bacterial pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus, and other infectious agents affecting pigs. Because pigs share many physiological and immunological similarities with humans, swine CD40L is also studied in comparative immunology, vaccine development, and translational animal models, helping to improve understanding of costimulatory signaling and immune regulation in both veterinary and human medicine.
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