Swine GM-CSF Recombinant Protein

Catalog Number:: RP0940S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Sus scrofa (pig)

Swine GM-CSF (catalog RP0940S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.4 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and myeloid-cell biology (including proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	APTRPPSPVT RPWQHVDAIK EALSLLNNSN DTAAVMNETV DVVCEMFDPQ EPTCVQTRLN LYKQGLRGSL TRLKSPLTLL AKHYEQHCPL TEETSCETQS ITFKSFKDSL NKFLFTIPFD CWGPVKK (127)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	14.4 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Swine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In pigs (Sus scrofa), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Swine GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, while enhancing their phagocytic activity and antigen-presenting capacity. In swine immunity, GM-CSF contributes to regulation of innate and adaptive immune responses, particularly during viral and bacterial infections affecting the respiratory and gastrointestinal systems. Pigs are widely used as translational animal models for human infectious disease and vaccine research, and GM-CSF-mediated immune responses have been studied in porcine models of influenza infection, where activation of macrophages and dendritic cells helps coordinate antiviral immunity and inflammatory responses, providing insights relevant to both swine health and human respiratory disease research.

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