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Canine CD40 Ligand (TNFSF5) Recombinant Protein
- Catalog Number:
- RP0941D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine CD40 Ligand (CD40L, CD154, TNFSF5) (catalog RP0941D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.8 kDa, 149 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CD40L/CD154 signaling and immune-cell activation (including regulation of B-cell proliferation, antibody production, and T-cell interactions); typical experimental uses include cell-culture stimulation and dosing studies, activation and signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MQKGDQDPRI AAHVISEASS NPASVLRWAP KGYYTISSNL VSLENGKQLA VKRQGLYYVY AQVTFCSNRA ASSQAPFVAS LCLHSPSGTE RVLLRAASSR GSSKPCGQQS IHLGGVFELH PGASVFVNVT DPSQVSHGTG FTSFGLLKL (149) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 15.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine CD40 Ligand (CD40L), also known as CD154 and encoded by the CD40LG gene, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and cell-cell communication. In dogs (Canis lupus familiaris), CD40L is primarily expressed on activated CD4⁺ T lymphocytes, and can also be detected on platelets and other activated immune cells following antigen stimulation. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, triggering intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, immunoglobulin class switching, germinal center formation, and the development of memory B cells. Through these mechanisms, canine CD40L plays a key role in T cell-dependent antibody responses and regulation of adaptive immunity. In canine health, the CD40-CD40L pathway contributes to immune responses against viral, bacterial, and parasitic pathogens, and dysregulation of this signaling axis has been implicated in immune-mediated diseases and certain cancers. Because dogs develop several naturally occurring diseases that resemble human conditions, canine CD40L is also studied in comparative animal models of immune-mediated disease, cancer immunology, and vaccine responses, helping to advance understanding of costimulatory signaling and adaptive immune regulation in both veterinary and human medicine.
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