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Multi-species (Ovine, Caprine) IL-21 Recombinant Protein
- Catalog Number:
- RP0954V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Budorcas taxicolor (takin), Capra hircus (goat), Oryx dammah (scimitar-horned oryx), Ovis aries (sheep)
- Caprine/Ovine IL-21 (catalog RP0954V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.2 kDa, 129 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat, sheep, scimitar-horned oryx, and takin. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-21 signaling and immune-cell function (including regulation of T-cell, B-cell, and NK-cell activity); typical experimental uses include cell-culture stimulation and dosing studies, signaling and functional assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | HKSSFQGQDR LFIRLRQLID IVDQLKNYVN DLDPEFLPAP EDVKRHCERS AFSCFQKVQL KSANNGDNEK IINILTKQLK RKLPPTNAGR RQKHELTCPS CDSYEKKPPK EYLERLKSLI QKMIHQHLS (129) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 15.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine IL-21 (Interleukin-21) is a cytokine of the common γ chain (γc) family that plays a central role in regulating adaptive immune responses in sheep (Ovis aries). IL-21 is produced primarily by activated CD4⁺ T cells, particularly T follicular helper (Tfh) cells and Th17 cells, following antigen stimulation. It signals through the IL-21 receptor (IL-21R) in association with the common γ chain, activating downstream JAK/STAT pathways-predominantly STAT3-to regulate gene expression in B cells, T cells, and natural killer (NK) cells. IL-21 promotes B cell proliferation, plasma cell differentiation, immunoglobulin class switching, and antibody production, while enhancing CD8⁺ T cell cytotoxic activity and NK cell function. In healthy sheep, basal IL-21 expression is generally low but increases during infection, vaccination, and immune activation. Ovine IL-21 has been implicated in immune responses to pathogens such as ovine lentivirus (maedi-visna), Mycobacterium avium subspecies paratuberculosis, gastrointestinal nematodes (e.g., Haemonchus contortus), and respiratory pathogens. In veterinary and agricultural research, ovine IL-21 serves as a biomarker of T cell-dependent B cell help and humoral immunity and is studied in the context of vaccine efficacy, host-pathogen interactions, and regulation of adaptive immune responses in small ruminant health and production systems.
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