Bovine CCL3 (MIP-1 alpha) Recombinant Protein

Catalog Number:: RP0974B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle)

Bovine CCL3 (MIP-1α) (catalog RP0974B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, American buffalo, zebu, banteng, and wild yak. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of macrophages, NK cells, and T cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	APFGADTPTA CCFSYVARQL SRKIVADYFE TSSQCSKPGV IFQTKKGRQV CANPTEDWVQ EYITDLELNA (70)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	7.8 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in cattle (Bos taurus). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, natural killer (NK) cells, dendritic cells, and activated T lymphocytes to sites of infection or tissue injury. In cattle, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment plays an important role in host defense against pathogens involved in diseases such as bovine respiratory disease complex (BRDC), mastitis, and mycobacterial infections including Mycobacterium bovis. While CCL3-driven leukocyte trafficking supports pathogen clearance and initiation of immune responses, excessive or prolonged expression may contribute to inflammatory tissue damage and chronic inflammation. As a regulator of immune cell migration and inflammatory signaling, bovine CCL3 is widely studied in investigations of host-pathogen interactions, inflammatory disease mechanisms, and vaccine responses aimed at improving disease resistance and health in cattle.

Choose a size

Please choose a size to see price

RELATED MEDIA