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Rabbit GM-CSF Recombinant Protein
- Catalog Number:
- RP0977U
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Oryctolagus cuniculus (rabbit)
- Rabbit GM-CSF (catalog RP0977U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.4 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and myeloid-cell biology (including proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APTHQPNTVS QPLKHVDAIK EARIILSRSN DSATVLGEMV EVVSEMFDPQ KPTCLQTRLE LYKQGLRGSL ERLSSTLTLM ASHYKQNCPP TPETSCETEF ITFKSFKENL KCFLFVIPFN CWEPVQK (127) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 14.4 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rabbit GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In rabbits (Oryctolagus cuniculus), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Rabbit GM-CSF binds to the GM-CSF receptor (CSF2R) on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, while enhancing their phagocytic and antigen-presenting functions. In rabbit immunity, GM-CSF contributes to innate immune responses and inflammatory regulation, particularly through activation of neutrophils and macrophages during infection. Rabbits are also widely used as animal models for studying inflammatory and infectious diseases, and GM-CSF-mediated immune responses have been examined in rabbit models of bacterial pneumonia, where enhanced macrophage and neutrophil activation contributes to improved pathogen clearance and regulation of lung inflammation, providing insights relevant to host defense mechanisms and therapeutic strategies for respiratory infections.
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