Dolphin IL-10 Recombinant Protein

Catalog Number:
RP0978P
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Tursiops truncatus (common bottlenose dolphin)
  • Dolphin IL-10 (catalog RP0978P) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.6 kDa, 160 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dolphin. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-10 signaling and immune regulation (including anti-inflammatory responses and cytokine suppression); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGQHQGTQAKD SCIHFPDSLP HMLRELRAAF SSVKTFFQMN DQLDNSLLSQ SLLEDFKGYL GCQALSEMIQ FYLEEVMPKA EDHGPNIKEH VNSLGEKLKT LRLQLRRCHR FLPCENKSKA VEQVKSVFNK LQEKGVYKAM SEFDIFINYI EAYMITKMKN (160)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight18.6 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids160
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Dolphin Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced by regulatory T cells, macrophages, dendritic cells, and certain B-cell populations in dolphins, particularly the bottlenose dolphin (Tursiops truncatus), where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, TNF-α, and IL-6), reduce antigen presentation, and downregulate Th1-type immune responses. In dolphin health, IL-10 is important in modulating inflammatory responses associated with viral infections such as cetacean morbillivirus and herpesvirus, bacterial pathogens including Brucella spp., chronic dermatitis, pneumonia, and wound healing, where balanced IL-10 expression helps prevent tissue damage while supporting pathogen control. Dysregulated IL-10 responses may reflect immune suppression linked to environmental stressors such as pollutant exposure or harmful algal blooms. As dolphins serve as sentinel species for marine ecosystem health, IL-10 is a valuable biomarker of immune regulation and resilience in both free-ranging and managed populations. In comparative and conservation research, characterization of dolphin IL-10 supports studies of immune tolerance, host–pathogen interactions in marine mammals, environmental immunotoxicology, and cytokine network balance, providing insight relevant to wildlife health and broader mammalian immunology.
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