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Canine CCL3 (MIP-1 alpha) Recombinant Protein
- Catalog Number:
- RP0980D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus familiaris (dog)
- Canine CCL3 (MIP-1α) (catalog RP0980D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.1 kDa, 73 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of macrophages, NK cells, and T cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | QVFSPFGADT PIACCFSYVS KQIPRKFIVD CFETSSQCSK PGIIFETRKG RQACANPSEA WVQEYVADLK LKA (73) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.1 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that plays a key role in recruitment and activation of immune cells during inflammatory and infectious responses in dogs (Canis lupus familiaris). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In dogs, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial products and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment contributes to host defense in infectious diseases and inflammatory conditions such as canine leishmaniasis, bacterial infections, and immune-mediated inflammatory disorders, where monocyte and T cell infiltration supports pathogen clearance and immune activation. However, excessive or prolonged CCL3 expression may contribute to chronic inflammation and tissue damage. CCL3 is also studied in canine cancers such as lymphoma and mast cell tumors, where chemokine-driven immune cell infiltration can influence tumor microenvironment dynamics. As a regulator of leukocyte trafficking and inflammatory signaling, canine CCL3 is important for studies of host-pathogen interactions, immune regulation, and disease pathogenesis in both veterinary and comparative biomedical research.
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