Slide 1 of 1 
Canine CX3CL1 (Fractalkine) Recombinant Protein
- Catalog Number:
- RP0985D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine CX3CL1 (Fractalkine) (catalog RP0985D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.7 kDa, 76 amino acids long (chemokine domain; full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CX3CL1/fractalkine signaling and leukocyte trafficking (including adhesion and migration of monocytes, NK cells, and T cells); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | QHLGVKKCNV TCHKMTSEIP VALLVHYQRN QESCGKPAII LKTKQNRIFC ADPKERWVQK AIAHLEHQIA VTIRNG (76) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.7 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine CX3CL1 (C-X3-C motif chemokine ligand 1), also known as fractalkine, is a chemokine belonging to the CX3C chemokine family, characterized by three amino acids separating the first two conserved cysteine residues. CX3CL1 is unique among chemokines because it exists in both a membrane-bound form and a soluble form, allowing it to function in both leukocyte adhesion and chemotaxis. In dogs (Canis lupus familiaris), CX3CL1 is produced primarily by endothelial cells, epithelial cells, neurons, and macrophages in response to infection, inflammatory cytokines, or tissue injury. CX3CL1 binds to its receptor CX3CR1, which is expressed on monocytes, macrophages, natural killer (NK) cells, and subsets of T lymphocytes, promoting adhesion, migration, and activation of these immune cells at sites of inflammation. In canine health, CX3CL1 contributes to immune responses in inflammatory diseases, infectious conditions, and tissue injury, particularly in the central nervous system and cardiovascular tissues where fractalkine signaling regulates leukocyte-endothelial interactions. Dogs also serve as comparative animal models for human inflammatory and neurological diseases, and the CX3CL1-CX3CR1 pathway has been studied in canine models of neuroinflammation and spinal cord injury, where fractalkine signaling influences microglial activation and immune cell recruitment in damaged neural tissue. These studies help improve understanding of chemokine-mediated neuroimmune interactions and inflammatory mechanisms relevant to both veterinary medicine and human neurological disease research.
Please choose a size to see price
RELATED MEDIA
