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Feline CD40 Ligand (TNFSF5) Recombinant Protein
- Catalog Number:
- RP0988F
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Acinonyx jubatus (cheetah), Felis catus (domestic cat), Lynx canadensis (Canada lynx), Lynx pardinus (Spanish lynx), Panthera pardus (leopard), Panthera tigris altaica (Amur tiger)
- Feline CD40 Ligand (CD40L, CD154, TNFSF5) (catalog RP0988F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.9 kDa, 149 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, leopard, Amur tiger, cheetah, Canada lynx, and Spanish lynx. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CD40L/CD154 signaling and immune-cell activation (including regulation of B-cell proliferation, antibody production, and T-cell interactions); typical experimental uses include cell-culture stimulation and dosing studies, activation and signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MQKGDQDPRV AAHVISEASS STASVLQWAP KGYYTISSNL VTLENGKQLA VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLHSPSGSE RVLLRAANAR SSSKPCGQQS IHLGGVFELH PGASVFVNVT DPSQVSHGTG FTSFGLLKL (149) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 15.9 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Feline CD40 Ligand (CD40L), also known as CD154 and encoded by the CD40LG gene, is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and cell-cell signaling. In cats (Felis catus), CD40L is primarily expressed on activated CD4⁺ T lymphocytes and can also be found on platelets and other immune cells following activation. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, triggering intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, immunoglobulin class switching, germinal center formation, and development of memory B cells. Through these interactions, feline CD40L plays a critical role in T cell-dependent antibody responses and coordination of adaptive immunity. In feline health, the CD40-CD40L signaling pathway is important for immune responses to viral infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) as well as bacterial and parasitic pathogens. Because CD40L is essential for T-cell-mediated B-cell help and immune regulation, feline CD40L is studied in veterinary immunology, infectious disease research, and vaccine development, helping to improve understanding of adaptive immune responses and immunomodulatory signaling in cats.
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