Canine IL-4 Recombinant Protein

Catalog Number:: RP0992D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus familiaris (dog)

Product Description

Canine IL-4 (catalog RP0992D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.8 kDa, 108 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-4 signaling and immune responses (including regulation of Th2 differentiation, B-cell activation, and antibody production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	HNFNITIKEI IKMLNILTAR NDSCMELTVK DVFTAPKNTS DKEIFCRAAT VLRQIYTHNC SNRYLRGLYR NLSSMANKTC SMNEIKKSTL KDFLERLKVI MQKKYYRH (108)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	12.8 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in dogs (Canis lupus familiaris), where it plays a central role in regulating humoral immunity, allergic inflammation, and alternative macrophage activation. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, IgE production, antibody class switching, and suppression of Th1-associated cytokines such as IFN-γ. In canine health, IL-4 is particularly important in allergic and atopic conditions including atopic dermatitis, food hypersensitivity, allergic otitis, and eosinophilic airway disease, where Th2 polarization drives IgE-mediated responses, pruritus, and chronic skin or airway inflammation. IL-4 also influences immune balance during parasitic infections and may affect Th1/Th2 dynamics in chronic infectious diseases. While IL-4 supports protective humoral responses and tissue repair, excessive or persistent IL-4 signaling can contribute to chronic allergic inflammation and barrier dysfunction. As a biomarker of type 2 immune activation, canine IL-4 is widely studied in allergy research, vaccine response evaluation, and immune modulation strategies. In translational research, dogs serve as valuable spontaneous models for human atopic dermatitis and allergic airway disease, and characterization of canine IL-4 supports comparative investigations into Th2-mediated inflammation and development of targeted cytokine therapies relevant to both veterinary and human medicine.

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