Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein

Catalog Number:: RP0997B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Product Description

Bovine LIF (Leukemia Inhibitory Factor) (catalog RP0997B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.8 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SPLPITPVNA TCATRHPCPS NLMNQIRNQL GQLNSSANSL FILYYTAQGE PFPNNLDKLC SPNVTDFPPF HANATEKARL VELYRIIAYL GASLGNITRD QKVLNPYAHG LHSKLSTTAD VLRGLLSNVL CRLCSKYHVS HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QVIAVLAQAF (180)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	19.8 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family-which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27-all of which signal through receptor complexes containing the shared gp130 subunit. In cattle (Bos taurus), LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K signaling pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. Bovine LIF plays important roles in reproductive biology, particularly in endometrial function, embryo implantation, and early embryonic development, making it highly relevant to fertility and reproductive management in dairy and beef production systems. LIF is also involved in hematopoiesis, neural cell survival, and modulation of inflammatory responses during infections such as mastitis and respiratory disease, where STAT3-driven signaling can influence tissue remodeling and immune regulation. Dysregulated LIF expression may contribute to fibrosis or tumor progression in certain contexts. As both a reproductive and immunomodulatory cytokine, bovine LIF is important in studies of fertility, embryo culture systems, stem cell biology, and cytokine network regulation. Characterization of bovine LIF supports veterinary research aimed at improving reproductive efficiency, understanding inflammatory disease mechanisms, and advancing regenerative and translational applications relevant to livestock and comparative medicine.

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