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Canine Leukemia Inhibitory Factor (LIF) Recombinant Protein
- Catalog Number:
- RP1010D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine LIF (Leukemia Inhibitory Factor) (catalog RP1010D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.7 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog, dingo, Arctic fox, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SPLPITPVNA TCATRHPCHS NLMNQIRNQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANSTEKTRL VELYRIIAYL GASLGNITRD QKVLNPNALS LHSKLNATAD IMRGLLSNVL CRLCSKYHVA HVDVAYGPDT SGKDVFQKKK LGCQLLGKYK QVIAVVAQAF (180) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 19.7 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family-which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27-all of which signal through receptor complexes containing the shared gp130 subunit. In dogs (Canis lupus familiaris), LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K signaling pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. Canine LIF plays important roles in reproductive biology, particularly in endometrial receptivity and embryo implantation, as well as in hematopoiesis and neural cell survival. In canine health, LIF-mediated STAT3 signaling is relevant in inflammatory diseases, tissue regeneration, and oncology, where dysregulated LIF expression may contribute to tumor progression, tumor microenvironment remodeling, or fibrosis in cancers such as lymphoma, mammary tumors, and osteosarcoma. LIF may also influence immune balance during chronic inflammatory conditions such as inflammatory bowel disease. As both a regenerative and immunomodulatory cytokine, canine LIF is important in studies of fertility, stem cell biology, cancer pathogenesis, and cytokine network regulation. Given the dog's role as a spontaneous model for human cancer and chronic inflammatory disease, characterization of canine LIF supports translational research into IL-6 family signaling and development of targeted STAT3-modulating therapies relevant to both veterinary and human medicine.
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