Rabbit Leukemia Inhibitory Factor (LIF) Recombinant Protein

Catalog Number:
RP1011U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit LIF (Leukemia Inhibitory Factor) (catalog RP1011U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.7 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPLPINPVNA TCNTHHPCPS NLMSQIRSQL AQLNGTANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKVRL VELYRIVAYL GTALGNITRD QKTLNPTAHS LHSKLNATAD TLRGLLSNVL CRLCSKYHVA HVDVAYGPDT SGKDVFQKKK LGCQLLGKYK QVMAVLAQAF (180)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight19.7 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family—which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27—and signals through receptor complexes containing the shared gp130 subunit. In rabbits (Oryctolagus cuniculus), LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. Rabbit LIF plays important roles in reproductive biology, particularly in uterine receptivity and embryo implantation, and has been studied in the context of early embryonic development and stem cell maintenance. In disease models, LIF-mediated STAT3 signaling may influence inflammatory responses and tissue remodeling in experimental systems of atherosclerosis, osteoarthritis, ocular inflammation, pulmonary injury, and cancer. Dysregulated LIF expression could contribute to fibrosis or tumor progression through persistent STAT3 activation. Because rabbits are widely used as translational models in cardiovascular, musculoskeletal, and ophthalmic research, characterization of rabbit LIF supports investigations into IL-6 family cytokine signaling, regenerative biology, host–pathogen interactions, and development of STAT3-modulating therapeutic strategies relevant to human inflammatory and fibrotic diseases.

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