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Ovine IFN alpha Recombinant Protein
- Catalog Number:
- RP1027V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Ovis aries (sheep)
- Ovine IFN-α (catalog RP1027V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.0 kDa, 166 amino acids long (full sequence provided; variant [Ala75Thr], [Ser164Arg]), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study type I interferon signaling and antiviral immune responses (including regulation of innate immunity and gene expression); typical experimental uses include cell-culture stimulation and antiviral assays, signaling and gene-expression studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | CHLPHTHSLA NRRVLTLLRQ LRRVSPSSCL QDRKDFAFAQ EVLGGSQLRK VQAISVLHEV TQHTFQLFST EGSATAWDQS LLDKLRAALD QQLTDLQACL RQEEGLRGAP LLKEDSSLAV RKYFHRVTLY LQEKGHSPCA WEVVRAEVMR AFSSSTNLQE RFRRKD (166) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 19.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine Interferon-Alpha (IFN-α) is a type I interferon produced primarily by plasmacytoid dendritic cells and virus-infected cells in sheep (Ovis aries), where it plays a central role in early innate antiviral immunity. Upon binding to the type I interferon receptor complex (IFNAR1/IFNAR2), ovine IFN-α activates JAK/STAT signaling pathways, leading to induction of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. Ovine IFN-α is critically involved in host defense against economically significant viral infections such as bluetongue virus (BTV), ovine progressive pneumonia virus (maedi-visna; a small ruminant lentivirus), peste des petits ruminants virus (PPRV), and sheep pox virus. Viral modulation or evasion of type I interferon responses can contribute to persistence, chronic inflammation, or systemic disease. The magnitude and timing of IFN-α induction influence disease outcome, viral shedding, and vaccine responsiveness in sheep populations. In veterinary and comparative research, characterization of ovine IFN-α supports studies of lentiviral pathogenesis, arboviral disease, mucosal antiviral immunity, and type I interferon biology in ruminants, contributing to improved disease control strategies and broader understanding of antiviral immune mechanisms relevant to both animal and human health.
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