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Caprine TNF alpha Recombinant Protein
- Catalog Number:
- RP1036G
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Capra hircus (goat)
- Caprine TNF-α (catalog RP1036G) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.2 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TNF-α signaling and inflammatory responses (including regulation of immune-cell activation, apoptosis, and cytokine production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | LRSSSQASSN KPVAHVVANI SAPGQLRWGD SYANALKANG VELKDNQLVV PTDGLYLIYS QVLFRGHGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEGAE AKPWYEPIYQ GGVFQLEKGD RLSAEINQPE YLDYAESGQV YFGIIAL (157) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Caprine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in goats (Capra hircus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins involved in immune signaling, apoptosis, and inflammatory regulation. In goats, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In caprine health, TNF-α contributes to immune responses against bacterial infections such as mastitis pathogens, Mycobacterium avium subsp. paratuberculosis (Johne's disease), and respiratory pathogens, where it promotes macrophage activation and inflammatory defense mechanisms. While TNF-α is essential for pathogen clearance, excessive or prolonged TNF signaling may contribute to chronic inflammation and tissue damage. Because TNF-α is a key mediator of inflammatory and immune responses, caprine TNF-α is studied in veterinary immunology, infectious disease research, and mastitis biology, contributing to improved understanding of immune regulation and disease resistance in goats.
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