Zebrafish VEGF-A Recombinant Protein

Catalog Number:
RP1040Z
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Danio rerio (zebrafish)
  • Zebrafish VEGF-A (catalog RP1040Z) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.0 kDa, 121 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to zebrafish. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study VEGF-A signaling and angiogenesis (including endothelial-cell proliferation, migration, and vascular development); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and migration assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAHIPKEGGKS KNDVIPFMDV YKKSACKTRE LLVDIIQEYP DEIEHTYIPS CVVLMRCAGC CNDEALECVP TETRNVTMEV LRVKQRVSQH NFQLSFTEHT KCECRPKAEV KAKKERCEKP R (121)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.0 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids121
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Zebrafish (Danio rerio) Vascular Endothelial Growth Factor A (VEGF-A) is a member of the vascular endothelial growth factor (VEGF) family, which includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF)—key regulators of angiogenesis, vascular permeability, and endothelial cell growth. In zebrafish, VEGF-A is produced by multiple cell types including endothelial cells, macrophages, fibroblasts, epithelial cells, and developing embryonic tissues, particularly in response to hypoxia, inflammation, and tissue injury. VEGF-A exerts its biological effects primarily through binding to VEGF receptor homologs such as VEGFR-2 (Kdr/Kdrl) on endothelial cells, activating intracellular signaling pathways including MAPK/ERK, PI3K/AKT, and PLCγ, which promote endothelial cell proliferation, migration, vascular permeability, and formation of new blood vessels. In zebrafish, VEGF-A plays essential roles in embryonic vascular development, organogenesis, wound healing, and tissue regeneration, including regeneration of fins, heart tissue, and other organs. Because zebrafish embryos are transparent and genetically tractable, VEGF-A signaling has been extensively studied using live imaging and genetic models to investigate angiogenesis, vascular patterning, and responses to hypoxia or injury. As a result, zebrafish VEGF-A serves as an important model for understanding vertebrate vascular development and angiogenic signaling pathways relevant to human cardiovascular disease, cancer, and regenerative medicine.
  • Growth Factor
  • VEGF Superfamily

Please choose a size to see price


Reagents for Animal Model and Animal Health Research

Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

Animal Antibodies

Newsletter

"