Rabbit CXCL13 Recombinant Protein

Catalog Number:
RP1057U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit CXCL13 (catalog RP1057U) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.5 kDa, 83 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL13 signaling and immune-cell trafficking (including B-cell homing to lymphoid follicles); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVLEAYNTDLK CECVQETANY IPIRFIDRLQ IVPPGNGCPK REVIVWLKTK IAVCVKPHAK WLQKLIQILQ KKTMLSTPPA PVI (83)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight9.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit CXCL13 (C-X-C motif chemokine ligand 13), also known as BCA-1 (B-cell–attracting chemokine 1), is a homeostatic chemokine belonging to the CXC chemokine family that plays a central role in B-cell migration and organization of lymphoid tissues. In rabbits (Oryctolagus cuniculus), CXCL13 is produced primarily by follicular dendritic cells, stromal cells, and macrophages within secondary lymphoid organs, including the lymph nodes, spleen, and mucosal-associated lymphoid tissues. CXCL13 signals through the chemokine receptor CXCR5, which is expressed on B lymphocytes and T follicular helper (Tfh) cells, directing their migration into lymphoid follicles and germinal centers where antibody-producing B cells develop and undergo affinity maturation. In rabbit immunity, CXCL13 contributes to germinal center formation, B-cell homing, and development of humoral immune responses during infection and vaccination. Rabbits are widely used as animal models for immunology and antibody production, and CXCL13-mediated B-cell trafficking is important for understanding germinal center responses and antibody maturation in studies involving vaccine development and infectious disease research.

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