Feline Leukemia Inhibitory Factor (LIF) Recombinant Protein

Catalog Number:: RP1070F
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Acinonyx jubatus (cheetah), Callorhinus ursinus (northern fur seal), Eumetopias jubatus (Steller sea lion), Felis catus (domestic cat), Leopardus geoffroyi (Geoffroy's cat), Lynx canadensis (Canada lynx), Lynx pardinus (Spanish lynx), Lynx rufus (bobcat), Neofelis nebulosa (Clouded leopard), Odobenus rosmarus divergens (Pacific walrus), Panthera leo (lion), Panthera onca (jaguar), Panthera pardus (leopard), Panthera tigris (tiger), Panthera tigris altaica (Amur tiger), Panthera uncia (snow leopard), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma), Zalophus californianus (California sea lion)

Product Description

Feline LIF (Leukemia Inhibitory Factor) (catalog RP1070F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.7 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, cheetah, northern fur seal, Steller sea lion, Geoffroy's cat, Canada lynx, Spanish lynx, bobcat, clouded leopard, and Pacific walrus. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SPLPITPVNA TCATRHPCHS NLMNQIRNQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKTRL VELYRIIAYL GASLGNITRD QKVLNPNALS LHSKLNATAD IMRGLLSNVL CRLCNKYHVA HVDVAYGPDT SGKDVFQKKK LGCQLLGKYK QVIAVVAQAF (180)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	19.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Feline Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family-which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27-all of which signal through receptor complexes containing the shared gp130 subunit. In cats (Felis catus), LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K signaling pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. Feline LIF plays important roles in reproductive biology, particularly in embryo implantation and endometrial receptivity, as well as in hematopoiesis, neural cell survival, and inflammatory regulation. In feline health, LIF may contribute to modulation of inflammatory responses in chronic diseases such as inflammatory bowel disease and neoplasia, where STAT3-mediated signaling influences tissue remodeling and tumor microenvironment dynamics. Dysregulated LIF expression could impact fibrosis, immune tolerance, or tumor progression. As both an immunomodulatory and regenerative cytokine, feline LIF is relevant in studies of reproductive success, stem cell biology, oncology, and cytokine network regulation. Characterization of feline LIF supports comparative investigations into IL-6 family signaling pathways and their roles in inflammation, tissue repair, and disease pathogenesis in both veterinary and human medicine.

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