Cynomolgus Monkey Leukemia Inhibitory Factor (LIF) Recombinant Protein

Catalog Number:
RP1074Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Colobus angolensis palliates (Angolan Colobus), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Papio anubis (olive baboon)
  • Cynomolgus Monkey LIF (Leukemia Inhibitory Factor) (catalog RP1074Y) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.7 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, sooty mangabey, green monkey, Angolan colobus, rhesus monkey, pig-tailed macaque, drill, and olive baboon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK ETIAVLAQAF (180)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight19.7 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family—which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27—and signals through receptor complexes containing the shared gp130 subunit. Highly homologous to human LIF, cynomolgus LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K signaling pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. In nonhuman primates, LIF plays important roles in reproductive biology, including endometrial receptivity and embryo implantation, as well as in hematopoiesis, neural cell maintenance, and stem cell regulation. LIF-mediated STAT3 signaling is also involved in modulation of inflammatory responses and tissue remodeling during infectious disease models (e.g., dengue, influenza, SARS-CoV-2) and in studies of fibrosis and cancer biology. Dysregulated LIF expression may contribute to tumor progression or fibrotic pathology, making it relevant in oncology and safety pharmacology research. Because cynomolgus monkeys are widely used in preclinical evaluation of biologics and immune-modulating therapies, characterization of LIF supports translational investigations into IL-6 family signaling, STAT3-targeted therapeutics, reproductive medicine, and inflammatory disease mechanisms relevant to human health.

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