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Rat Leukemia Inhibitory Factor (LIF) Recombinant Protein
- Catalog Number:
- RP1076R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat LIF (Leukemia Inhibitory Factor) (catalog RP1076R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.8 kDa, 180 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study LIF signaling and cellular differentiation and survival (including maintenance of stem cell pluripotency and regulation of cell growth and inflammation); typical experimental uses include cell-culture stimulation and dosing studies, signaling and differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVAYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSNVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISAVVQAF (180) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 19.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the IL-6 family-which includes IL-6, IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27-and signals through receptor complexes containing the shared gp130 subunit. In rats (Rattus norvegicus), LIF binds to the LIF receptor (LIFR) in association with gp130, activating JAK/STAT3, MAPK, and PI3K signaling pathways that regulate cell survival, differentiation, immune modulation, and tissue repair. Rat LIF plays important roles in reproductive biology, particularly in embryo implantation and uterine function, as well as in hematopoiesis and neural development. In experimental models, LIF-mediated STAT3 signaling is involved in neuroprotection following brain injury, modulation of inflammatory responses in colitis and lung injury, cardiac remodeling after ischemia, and regulation of tumor growth and fibrosis. Dysregulated LIF expression may contribute to chronic inflammation or pathological tissue remodeling through sustained STAT3 activation. Because rats are widely used in neuroscience, cardiovascular, toxicology, and pharmacology research, characterization of rat LIF supports investigations into IL-6 family cytokine signaling, regenerative mechanisms, inflammatory disease pathways, and development of STAT3-targeted therapeutic strategies relevant to human medicine.
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