Mouse CXCL5 (ENA-78) Recombinant Protein

Catalog Number:
RP1084M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus musculus (house mouse)
  • Mouse CXCL5 (catalog RP1084M) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.9 kDa, 92 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL5 signaling and immune-cell chemotaxis (including neutrophil recruitment and activation); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ (92)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight9.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse CXCL5 (C-X-C motif chemokine ligand 5), also known as LIX (lipopolysaccharide-induced CXC chemokine), is a proinflammatory chemokine belonging to the ELR⁺ CXC chemokine family, which includes CXCL1 (KC), CXCL2 (MIP-2), and CXCL3 that regulate neutrophil recruitment during inflammatory responses. In mice (Mus musculus), CXCL5 is produced by macrophages, epithelial cells, endothelial cells, fibroblasts, and other tissue cells in response to bacterial products such as lipopolysaccharide (LPS), proinflammatory cytokines including IL-1β and TNF-α, and tissue injury. CXCL5 signals primarily through the chemokine receptor CXCR2, expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. In murine immunology, CXCL5 plays an important role in innate immune defense and inflammatory signaling, particularly in the lung and gastrointestinal tract. Mouse CXCL5 has been extensively studied in experimental models of acute lung injury and bacterial pneumonia, where CXCL5-mediated recruitment of neutrophils contributes to pulmonary inflammation and host defense against respiratory pathogens. Because mice are widely used for genetic and experimental models of inflammatory disease, CXCL5 has been important for understanding neutrophil trafficking, chemokine signaling, and mechanisms of inflammatory tissue injury and host protection.

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