Cynomolgus Monkey IL-2 Recombinant Protein

Catalog Number:
RP1096Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Macaca fascicularis (crab-eating macaque)
  • Cynomolgus Monkey IL-2 (catalog RP1096Y) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.5 kDa, 134 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-2 signaling and T-cell biology (including T-cell proliferation, activation, and survival); typical experimental uses include cell-culture stimulation and dosing studies, proliferation and activation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELRHLQCLE EELKPLEEVL NLAQSKSFHL RDTKDLISNI NVIVLELKGS ETTLMCEYAD ETATIVEFLN RWITFCQSII STLT (134)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight15.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus monkey IL-2 (Interleukin-2) is a central T cell–derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in Cynomolgus monkeys (Macaca fascicularis). The IL-2 protein is highly conserved among non-human primates and is functionally comparable to that of Rhesus monkey (Macaca mulatta) and humans, supporting its translational relevance in preclinical research. IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ, and the common γ chain) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy Cynomolgus monkeys, basal IL-2 expression is typically low but increases during infection, vaccination, or immune stimulation. Cynomolgus monkeys serve as important preclinical models for human infectious diseases, autoimmune disorders, transplant biology, and immuno-oncology. In translational and safety pharmacology studies, IL-2 is used as a biomarker of T cell activation and cell-mediated immunity and is particularly relevant in evaluation of biologics, vaccines, and immunotherapies targeting T cell pathways.

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