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Canine RANKL (TNFSF11) Recombinant Protein
- Catalog Number:
- RP1106D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine RANKL (Receptor Activator of NF-κB Ligand, TNFSF11) (catalog RP1106D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.8 kDa, 175 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog, dingo, red fox, and Arctic fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study RANKL signaling and bone metabolism (including regulation of osteoclast differentiation and activation); typical experimental uses include cell-culture stimulation and differentiation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | EKAMMEGSWL EMARRGKTHT QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYFLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VLDID (175) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 19.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine RANKL (Receptor Activator of Nuclear Factor κB Ligand, also known as TNFSF11, TRANCE, or OPGL) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL that regulate immune signaling, apoptosis, and cellular differentiation. In dogs (Canis lupus familiaris), RANKL is expressed primarily by osteoblasts, bone marrow stromal cells, activated T lymphocytes, and other immune cells, and plays a key role in bone remodeling and immune regulation. RANKL functions by binding to its receptor RANK (receptor activator of NF-κB) on osteoclast precursors and mature osteoclasts, activating intracellular signaling pathways including NF-κB, MAPK, and NFATc1, which promote osteoclast differentiation, activation, and bone resorption. The activity of RANKL is tightly controlled by osteoprotegerin (OPG, TNFRSF11B), a soluble decoy receptor that binds RANKL and prevents its interaction with RANK, thereby regulating bone turnover. In canine health, the RANKL-RANK-OPG signaling axis is important for skeletal development, bone remodeling, and calcium homeostasis, and dysregulation of this pathway has been associated with bone loss disorders, periodontal disease, and osteolytic processes associated with bone tumors such as osteosarcoma. Because dogs are also used as comparative models for human bone diseases and osteosarcoma, canine RANKL is studied in veterinary orthopedics, oncology, and bone biology research, contributing to understanding of osteoclast regulation and skeletal disease.
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