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Swine C3a Recombinant Protein
- Catalog Number:
- RP1132S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine C3a (Complement Component 3a, Anaphylatoxin C3a) (catalog RP1132S) is a yeast-derived complement protein supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.1 kDa, 77 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study complement activation and inflammatory responses (including anaphylatoxin-mediated signaling, immune-cell recruitment, and vascular permeability); typical experimental uses include cell-culture stimulation and signaling assays, inflammation studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SVQLMEKRMD KLGQYSKDVR RCCEHGMRDN PMKFSCQRRA QFIQHGDACV KAFLDCCEYI AKLRQQHSRN KPLGLAR (77) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 9.1 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine C3a is a biologically active anaphylatoxin generated during activation of the complement cascade in pigs (Sus scrofa). It is produced through proteolytic cleavage of complement component C3 and functions as a potent mediator of innate immune and inflammatory responses. C3a exerts its effects by binding to the C3a receptor (C3aR) expressed on immune cells such as neutrophils, monocytes, macrophages, mast cells, and certain epithelial and endothelial cells, leading to chemotaxis, cytokine release, increased vascular permeability, smooth muscle contraction, and amplification of inflammatory signaling. In healthy swine, circulating C3a levels are typically low but increase rapidly during complement activation triggered by infection, immune complex formation, tissue injury, endotoxemia, or vaccine-induced immune responses. In veterinary and translational research, swine C3a is particularly relevant in models of sepsis, ischemia-reperfusion injury, acute lung injury, xenotransplantation, and inflammatory diseases, as pigs share important physiological similarities with humans. Measurement and characterization of swine C3a provide valuable insight into complement activation, systemic inflammation, and innate immune regulation in both agricultural and biomedical research settings.
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