Atlantic Salmon IL-21 Recombinant Protein

Catalog Number:
RP1178AS
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Salmo salar (Atlantic salmon)
  • Atlantic Salmon IL-21 (Interleukin-21) (catalog RP1178AS) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.2 kDa, 122 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Atlantic salmon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-21 signaling and immune responses (including regulation of lymphocyte function and cytokine production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceDNVERTMKLT EVLKELRQLN KSVEHNGVML NTPTMDIEEC CFLSTLECFQ KMVPRLKTKQ KKLQCKVSRN LKPLTFTGVD SCSREEREKK VCQGCDSYPM KDSREFVQQL ESLLQKAMTR LV (122)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids122
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Atlantic salmon IL-21 (Interleukin-21) is a cytokine of the common γ chain (γc) family that plays an important role in regulation of adaptive immune responses in Atlantic salmon (Salmo salar). IL-21 is produced primarily by activated T lymphocytes, including CD4-like T cells, following antigen stimulation. It signals through the IL-21 receptor (IL-21R) in association with the common γ chain, activating downstream JAK/STAT pathways—particularly STAT3—to regulate gene expression in target immune cells. In teleost fish, IL-21 promotes B cell proliferation, differentiation, and immunoglobulin production, and supports cytotoxic T cell responses, contributing to both humoral and cell-mediated immunity. In healthy Atlantic salmon, basal IL-21 expression is generally low but increases during infection, vaccination, and immune stimulation. IL-21 has been implicated in host responses to important aquaculture pathogens such as infectious salmon anemia virus (ISAV), piscine orthoreovirus (PRV), infectious pancreatic necrosis virus (IPNV), and bacterial infections including Aeromonas salmonicida. In fish immunology and aquaculture research, Atlantic salmon IL-21 serves as a biomarker of T cell–dependent adaptive immunity and vaccine responsiveness, providing insight into host–pathogen interactions and immune regulation in economically important marine species.
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Fax: 651-646-0095

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