Ovine GM-CSF Recombinant Protein

Catalog Number:: RP1190V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Ovis aries (sheep)

Ovine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) (catalog RP1190V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.4 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and hematopoiesis (including regulation of granulocyte and macrophage differentiation and activation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	APTRQPSPVT RPWQHVDAIK EALSLLNDST DTAAVMDETV EVVSEMFDSQ EPTCLQTRLE LYKQGLRGSL TSLTGSLTMM ASHYKKHCPP TQETSCETQI ITFKSFKENL KDFLFIIPFD CWEPVQK (127)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	14.4 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Ovine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In sheep (Ovis aries), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Ovine GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, while enhancing their phagocytic and antigen-presenting functions. In ovine immunity, GM-CSF contributes to host defense against bacterial and parasitic infections, particularly through activation of macrophages and neutrophils in tissues such as the lungs and gastrointestinal tract. Sheep are also used as animal models for studying respiratory and inflammatory diseases, and GM-CSF-regulated myeloid responses have been investigated in ovine models of respiratory syncytial virus (RSV) infection, which closely resemble human infant RSV disease, helping researchers understand immune mechanisms driving viral lung inflammation and evaluate potential therapeutic strategies.

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