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Rat IL-4 Recombinant Protein
- Catalog Number:
- RP1191R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat IL-4 (Interleukin-4) (catalog RP1191R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.8 kDa, 123 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-4 signaling and immune responses (including regulation of Th2 differentiation, B-cell activation, and antibody production); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | CNDSPLREII NTLNQVTEKG TPCTEMFVPD VLTATRNTTE NELICRASRV LRKFYFPRDV PPCLKNKSGV LGELRKLCRG VSGLNSLRSC TVNESTLTTL KDFLESLKSI LRGKYLQSCT SMS (123) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in rats (Rattus norvegicus), where it plays a central role in regulating humoral immunity, allergic inflammation, and alternative macrophage activation. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody production, IgE class switching, and suppression of Th1-associated cytokines such as IFN-γ. In rat models, IL-4 is widely studied in experimental systems of asthma, allergic airway inflammation, atopic dermatitis, parasitic infection, and autoimmune disease, where Th2 polarization drives eosinophilic infiltration, mucus hypersecretion, and tissue remodeling. IL-4 also influences macrophage polarization toward an M2 phenotype, contributing to wound healing and fibrotic responses in models of lung, liver, and cardiac fibrosis. While protective in supporting antibody-mediated immunity and parasite clearance, excessive or sustained IL-4 signaling can impair effective cell-mediated responses and promote chronic allergic pathology. As both a biomarker and mechanistic driver of type 2 immunity, rat IL-4 remains central to preclinical research on Th1/Th2 immune balance, cytokine regulation, and development of targeted therapies for allergic, fibrotic, and inflammatory diseases relevant to human health.
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